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2 article(s) from Kanavarioti, Anastassia

False positives and false negatives measure less than 0.001% in labeling ssDNA with osmium tetroxide 2,2’-bipyridine

Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 1434–1446, doi:10.3762/bjnano.7.135

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Scheme 1: A 1:1 mixture of osmium tetroxide with 2,2’-bipyridine in solution forms a reactive complex (OsBp),...

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Figure 1: (a) Reactivity profile of a 32-mer deoxyoligo, pGEX3’-A₉, in aqueous 12.6 mM OsBp at 25 °C monitore...

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Figure 2: Direct comparison of the kinetics of a 20 nucleotide long deoxyoligo (triangles, PCR primer “16S RN...

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Figure 3: HPLC analysis of deoxyoligos A₁₀XA₉ with X = A, G, C, T. An equimolar mixture of four 20-mers diffe...

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Figure 4: Overlapping HPLC profiles of A₂₀ before (red) and after (blue) osmylation to show no detectable rea...

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Figure 5: Overlapping HPLC profiles of A₁₀GA₉ before (red) and after (blue) osmylation to show no detectable ...

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Figure 6: Overlapping IE HPLC profiles of intact and osmylated oligo A₁₀TA₉ to show that the main peak (red) ...

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Figure 7: Magnification of Figure 6 to show the impurities present in A₁₀TA₉ and the fact that each oligo (red) that ...

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Figure 8: CE profiles of A₅GA₅GA₅GA₂, a 20-mer with 3 Gs, before and after extensive osmylation to show no de...

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Figure 9: Overlapping HPLC profiles of intact oligo and the corresponding product resulting by “super” osmyla...

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Figure 10: Overlapping CE profiles from stability studies of A₁₀C(OsBp)A₉ in water: Blue, after 2 h reaction i...

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Figure 11: CE profiles: (a) the two peaks corresponding to the two topoisomers of A₁₀C(OsBp)A₄ (C-oligo) by to...

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Figure 12: CE profiles: (a) after two days of incubation at room temperature a small shoulder appears at the m...

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Figure 13: HPLC profiles of weeks-old-aged osmylated oligos. C* is an abbreviation for C(OsBp) and T* is an ab...

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Full Research Paper

Published 12 Oct 2016

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Single pyrimidine discrimination during voltage-driven translocation of osmylated oligodeoxynucleotides via the α-hemolysin nanopore

Yun Ding and
Anastassia Kanavarioti

Beilstein J. Nanotechnol. 2016, 7, 91–101, doi:10.3762/bjnano.7.11

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Scheme 1: OsBp with dTMP. Reaction of osmium tetroxide with 2,2’-bipyridine forms a reactive complex (OsBp or...

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Figure 1: Strategy for sequencing DNA using the osmylated strands. All sequences shown refer to deoxybases; f...

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Figure 2: Reaction of 12.6 mM OsBp (the highest concentration of OsBp used under our conditions) with 20 μM dA...

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Figure 3: Capillary electrophoresis (CE) overlapping traces of oligodeoxynucleotides pGEX3’-dA₂₅ intact and p...

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Figure 4: Translocation time histograms for four different oligos: Three are oligodeoxyadenylates, dA₁₀XdA₉ w...

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Figure 5: Representation of osmylated DNA strands to illustrate the practically parallel line-up of the OsBp ...

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Figure 6: Heat plots, normalized residual current, I_r/I₀, vs translocation time, t (ms), for the deoxyoligos ...

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Figure 7: Sample I–t traces for the control dA₂₀ and for dA₂₅-pGEX3’ R1 (with 4T(OsBp)) to show (a) continuou...

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Full Research Paper

Published 22 Jan 2016

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